My research is focused on investigating the mechanism of root attachment and understanding carbon metabolism regulation. I have used a mariner-based transposon vector for insertion sequence mutagenesis in Rhizobium leguminosarum (1) to investigate the role of O2 in growth on glucose and succinate (2). Insertion sequencing has then been used as a method to investigate the mechanism of root attachment and colonization to legumes.
(1) Perry, B. J., & Yost, C. K. (2014). Construction of a mariner-based transposon vector for use in insertion sequence mutagenesis in selected members of the Rhizobiaceae. BMC microbiology, 14(1), 1.
(2) Wheatley, R. M., Ramachandran, V. K., Geddes, B. A., Perry, B. J., Yost, C. K., & Poole, P. S. (2017). Role of O2 in the Growth of Rhizobium leguminosarum bv. viciae 3841 on Glucose and Succinate. Journal of Bacteriology, 199(1), e00572-16.
In Oct 2016: DPhil Student, Year 3
This DPhil project is funded by the NERC